What Makes the JIS Z 2801 Test Method So Necessary For Businesses?

Frequently requested is a quantitative antimicrobial test according to Japanese Industrial Standard (JIS) Z 2801, which is checked with the International Organization for Standardization (ISO) 22196 and may be performed on plastics, foams, and textiles, among other things. When two common bacteria, E. coli, and Staphylococcus aureus, are inoculated into hard surfaces and plastics, and the results are seen, the JIS Z 2801 standard method is used to determine whether or not growth is possible. The usual period for testing is twenty-four hours. In terms of antibacterial activity and efficacy testing, the JIS Z 2801 antimicrobial surface test is one of the most often requested, fastest, and most reliable antimicrobial surface tests available.

The use of durability testing in combination with the JIS Z 2801 test method may provide crucial information on the antimicrobial efficacy when exposed to a range of environmental conditions such as heat, humidity, and UV radiation (amongst others).

Who is the target audience for the JIS Z 2801 test method?

  • It is necessary to remove the test microorganism straight from agar plates and dilute it in a buffer.
  • The concentration of the microbe is standardized via dilution in a nourishing broth.
  • Inoculate the control and test surfaces with 0.4 ml of the germ suspension in triplicate on both characters.
  • To avoid evaporation of the solution and guarantee intimate contact with the surface of the sample, the inoculum is next coated with a film.
  • Several live germs are measured by elution followed by dilution and plating immediately after injection of the reference sample.
  • T24 samples are incubated in a humid atmosphere for 24 hours after being collected from the other models.
  • Following incubation, the quantities of microorganisms on the surface are measured by elution, followed by dilution and plating.

The followings are some of the advantages of the JIS Z 2801 Test method

  • One of the first antimicrobial testing techniques developed.
  • JIS Z 2801 test method is a standard created for materials and goods intended for the Japanese market.
  • Antimicrobial tests performed following JIS standards are trustworthy and widely recognized throughout the world.
  • JIS Standards have been standardized to meet the needs of a wide variety of industries.
  • In comparison to JIS Z 2801 test method, ISO 22196 is a harmonized version of JIS Z 2801 that is almost similar to JIS Z 2801.

The following are requirements for JIS Z 2801:

  • The samples should have a 5 x 5 cm square footprint.
  • There is a minimum of 6 pieces of blank samples required for each measurement configuration.
  • A minimum of three parts of the antimicrobial-equipped models is needed for a measurement to be performed.
  • Smoothness and uniformity of the surface of the models are needed.
  • Liquid paints, colors, lacquers, and coatings are available for delivery.
  • Please bag the samples and label them with the information you want to convey in connection to the batch or series number, or the substance of the piece, as appropriate.
  • If you use felt pens or markers, please do not mark on the sample!
  • Because their colors dash, they are often inappropriate for use. This is true for both permanent markers and pens. If it becomes essential to label the sample, a pencil should be used.

The strengths of the JIS Z 2801 testing process

As long as the inoculum does not flow away from the target area after it has been coated with the thin film, this method is quantitative, and the results are often reproducible after the target region has been coated.

Apart from bacteriostatic (growth-inhibiting) and bactericidal (bacteria-killing) properties, it also has antimicrobial properties.

Because the microbial concentrations are standardized and bacteria are provided with nutrients during the incubation period, they have a longer length of time to grow if the surfaces are not sufficiently antimicrobial-resistant to bacteria. Some other antimicrobial experiments, in which bacteria are “incubated” in non-nutritive solutions for long periods, may be more stressful for the germs.

As part of the method, researchers conduct duplicate experiments, which enables them to evaluate the precision of individual tests while simultaneously improving the overall accuracy of the study.

According to the method, estimated levels of antimicrobial activity seen in test samples are classified as “pass” or “fail,”. Minimizing the amount of discretion needed in making antimicrobial activity judgments.

The implication of the JIS Z 2801 test

Although, as previously mentioned, the JIS Z 2801 is not necessarily representative of real-world surface contamination situations, it is nevertheless effective. A relatively dilute liquid microbial inoculum is spread over a wide surface area and then kept wet. In the vast majority of instances, microbiological contaminants attach to surfaces within minutes of contact. That is undesirable because it shortens the time that an aqueous medium is available to facilitate interaction between the antimicrobial surface and the bacteria. Consequently, JIS Z 2801 is used as a “best-case” scenario for testing for a wide range of products and services.

The fact that “control” polymers often show relatively moderate levels of antibacterial activity, in reality, makes identifying the most effective control surface a difficult challenge.

Examining and identifying the variables that influenced the test results

The method was united by reviewing the procedures that the various participants utilized. An extensive series of experiments revealed that four critical factors influence the outcome of antibacterial testing.

These are

  • Incubation time
  •  bacteria starting concentration
  •  physiological state of bacteria (stationary or exponential phase of growth)
  •  Nutrient concentration.

These factors are being investigated for their impact on testing conducted by ISO 22196 / JIS Z 2801.


A high level of quality testing is prioritized at Situ Biosciences’ product testing laboratory. The company believes that understanding project goals and communicating effectively are essential components of every successful project. Situ Biosciences’ antimicrobial laboratory can be used for fast and reliable antimicrobial testing to aid in product development and performance testing, and continuous quality control. Situ Biosciences’ antimicrobial laboratory can be used for fast and reliable antimicrobial testing to aid in product development.

Also, Read – 6 Ways to Use Technology In Small Businesses

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